Journal: Cells

Article Title: Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

doi: 10.3390/cells12172138

Figure Lengend Snippet: Immunohistochemical localization of SDF1 in dental pulp tissue cultured using the Improved CMDPT. ( a – d ) Immunohistochemical staining for SDF1 (( c , d ) are high magnification images of the red box area, respectively), ( e , f ) Negative control. ( a , c , e ) After 72 h of culture, ( b , d , f ). After 96 h of culture. SDF1 expression was observed in the center portion of the dental pulp tissue after 72 h of culture when active cell migration was observed. In contrast, no expression of SDF1 was observed at 96 h of culture, when almost all of the cells had migrated vertically toward the membrane. Arrowheads indicate the localization of SDF1. ( a – f ) Scale bars are 50 µm.

Article Snippet: After treatment with 10% normal goat serum at RT for 10 min, the sections were incubated with rabbit anti-Ki-67 antibody (Abcam, Cambridge, UK) (diluted 1:200 in PBS containing 1% bovine serum albumin) or rabbit anti-stromal cell-derived factor-1(SDF1) antibody (Abcam, Cambridge, UK) (diluted 1:1000 in PBS containing 1% bovine serum albumin) at 4 °C overnight.

Techniques: Immunohistochemistry, Cell Culture, Staining, Negative Control, Expressing, Migration, Membrane

Journal: Cells

Article Title: Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

doi: 10.3390/cells12172138

Figure Lengend Snippet: Effect of SDF1 on the migration of DPSCs in vitro ( a ) Photo of the culture insert. ( b ) The number of migrating cells. ( c ) The absorbance of crystal violet at 570 nm. A significant cell-migration-promoting effect of SDF1 was observed in the culture of DPSCs.

Article Snippet: After treatment with 10% normal goat serum at RT for 10 min, the sections were incubated with rabbit anti-Ki-67 antibody (Abcam, Cambridge, UK) (diluted 1:200 in PBS containing 1% bovine serum albumin) or rabbit anti-stromal cell-derived factor-1(SDF1) antibody (Abcam, Cambridge, UK) (diluted 1:1000 in PBS containing 1% bovine serum albumin) at 4 °C overnight.

Techniques: Migration, In Vitro

Journal: Cells

Article Title: Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

doi: 10.3390/cells12172138

Figure Lengend Snippet: Effect of SDF1-neutralizing antibody on the migration of DPSCs under Improved CMDPT. Histological images after 48 h ( a , d ), 72 h ( b , e ), 96 h ( c , f ) of cultures using the Improved CMDPT. ( a – f ) H&E staining. ( a – c ) Control. ( d – f ) SDF1-neutralizing antibody was added. After 48 h of culture, DPSCs were localized near the membrane in the culture without an SDF1-neutralizing antibody. In contrast, no cell migration was observed in the presence of SDF1-neutralizing antibody. After 72 h of culture, DPSCs migrated toward the membranes, whereas no migration was observed in the cultures containing SDF1-neutralizing antibody. After 96 h of culture, the cells in the control group, which had been localized in the vicinity of the membrane after 72 h of culture, were almost absent, presumably because of growth outside the dental pulp tissue. In contrast, in the SDF1-neutralizing-antibody-treated group, some DPSCs were localized near the upper and lower membranes. ( a – f ) Scale bars are 50 µm.

Article Snippet: After treatment with 10% normal goat serum at RT for 10 min, the sections were incubated with rabbit anti-Ki-67 antibody (Abcam, Cambridge, UK) (diluted 1:200 in PBS containing 1% bovine serum albumin) or rabbit anti-stromal cell-derived factor-1(SDF1) antibody (Abcam, Cambridge, UK) (diluted 1:1000 in PBS containing 1% bovine serum albumin) at 4 °C overnight.

Techniques: Migration, Staining, Membrane

Journal: Frontiers in Immunology

Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

doi: 10.3389/fimmu.2023.1114321

Figure Lengend Snippet: Immunohistochemistry of HND and non-HND facial skin specimens. (A) Representative histological image at 100x magnification (left) and 200x magnification (right). Quantified staining intensity of (B) SDF-1α, (C) IL-1β, (D) TGF-β, (E) TNF-α, and (F) VEGF. (Unpaired two-tailed t-test, SDF-1α; p = 0.0014, IL-1β; p = 0.0374, TGF-β; p = 0.0058, TNF-α; p = 0.0035, VEGF; p = 0.0002) HND, head and neck dermatitis; SDF-1 α , stromal cell-derived factor-1-alpha; IL-1 β , Interleukin-1-beta; TGF- β , transforming growth factor-beta; TNF- α , tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor. * : p<0.05; ** : p<0.01.

Article Snippet: Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam).

Techniques: Immunohistochemistry, Staining, Two Tailed Test, Derivative Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models

doi: 10.1155/2023/1381620

Figure Lengend Snippet: Summary of growth factors and extracellular matrix (ECM) used to examine the migratory behaviors of retinal progenitors cultured from Drosophila melanogaster (DPs) and from rodent (MPs).

Article Snippet: Stromal-derived growth factor 1-alpha (SDF1- α ; ThermoFisher Catalog #RP-8658) , 100 ng/mL , MPs.

Techniques: Cell Culture, Concentration Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models

doi: 10.1155/2023/1381620

Figure Lengend Snippet: Measurement of the chemotaxis of stem-like cells. (a) Numbers of motile Drosophila progenitors (DPs) exposed to concentration gradients of insulin ( N = 12), brain-derived neurotrophic factor (BDNF, N = 12), and pyramus (Pyr, N = 12) normalized to control (media only, N = 12). (b) Numbers of motile Mus progenitors (MPs) in response to concentration gradients of insulin-like growth factor 1 (IGF-1, N = 30), epidermal growth factor (EGF, N = 27), and stromal cell-derived factor 1-alpha (SDF-1 α , N = 28) and normalized to control (media only, N = 30). Bars indicate statistically significant increases compared to control ( ∗ p < 0.05).

Article Snippet: Stromal-derived growth factor 1-alpha (SDF1- α ; ThermoFisher Catalog #RP-8658) , 100 ng/mL , MPs.

Techniques: Chemotaxis Assay, Concentration Assay, Derivative Assay, Control

Journal: PLoS ONE

Article Title: FOXO4-Knockdown Suppresses Oxidative Stress-Induced Apoptosis of Early Pro-Angiogenic Cells and Augments Their Neovascularization Capacities in Ischemic Limbs

doi: 10.1371/journal.pone.0092626

Figure Lengend Snippet: (A) Representative fluorescence microscopic images of EPCs incorporated in endothelial cells of the ischemic limbs of athymic nude rats 60 h after intramuscular injection of EPCs, NT-EPCs, or FOXO4 KD -EPCs. Rat specific CD31-positive endothelial cells were stained red. Human specific PCNA-positive cells, which were defined as human-derived EPCs, were stained green. Scale bar: 50 μm. (B) Pooled data of the number of PCNA-positive EPCs incorporated in endothelial cells of the rat ischemic limbs 60 h after the injection of EPCs, NT-EPCs, or FOXO4 KD -EPCs (*: p<0.01; **: p<0.005; n = 5, each). (C) A representative western blotting photo of expressions of human specific VEGF, b-FGF, SDF-1, and IGF-1 in the rat ischemic limb 60 h after intramuscular injection of EPCs, NT-EPCs, or FOXO4 KD -EPCs (n = 4, each).

Article Snippet: The membrane was blocked with a buffer (Blocking One, Nacalai Tesque, Inc.) and incubated for 24 h at 4°C with primary antibodies as follows: a rabbit anti-human β-actin antibody, a rabbit anti-human FOXO3a antibody, a rabbit anti-human FOXO4 antibody, a rabbit anti-human Bim antibody, a rabbit anti-human cleaved caspase-3 antibody, a rabbit anti-human vascular endothelial growth factor (VEGF) antibody, a rabbit anti-human basic-fibroblast growth factor (b-FGF) antibody (above antibodies were purchased from Cell Signaling Technology), a rabbit anti-human stromal cell-derived factor-1 (SDF-1) antibody, and a rabbit anti-human insulin-like growth factor-1 (IGF-1) antibody (above antibodies were purchased from abcam).

Techniques: Fluorescence, Injection, Staining, Derivative Assay, Western Blot